|
KMID : 0378019740170040451
|
|
New Medical Journal
1974 Volume.17 No. 4 p.451 ~ p.455
|
|
|
Response of Thoracic Lymphocytes from Immunized Rabbits with Ehrlich Carcinoma to PHA Stimulation
|
|
이수영/Lee, Soo Young
장성근/최진/김영제/Chang, Sung Keun/Choe, Jin/Kim, Young Che
|
|
|
Abstract
|
|
|
Many collaborators in our laboratory investigated tumor immunity using transplantable Ehrlich carcinoma and they suggested that the immunity against Ehrlich carcinoma was mainly mediated by cell-mediated immune reaction in which thymus-dependent lymphocytes played a role.
It is a well known fact that phytohemagglutinin(PHA) stimulates the transformation of small I lymphocytes since Hungerford et al. (1959). Brostoff et al. (1969) demonstrated the lymphocytes responded to nonspecific stimuli such as PHA were all thymus-dependent lymphocytes (T-lymphocytes). Therefore we observed the percentage of blast-formed cells, tritiated thymidine labeled cells and P.A.S. positive cells of thoracic lymphocytes from normal or immunized rabbits with live or UV-irradiated Ehrlich ascites tumor cells following stimulation with PHA in vitro.
The results are as follows:
1. In a 3-day culture, percentages of blast-formed cells and labeled cells of all three groups are predominant, and the labeling indices are predominant in lymphocytes from immune rabbits.
2. Percentages of P. A. S. positive cells from normal or immune rabbit with live tumor cells are predominant in a 3-day culture, whereas that from immune rabbits with UV-irradiated tumor , cells is predominant in a 2-day culture.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|